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sf9 insect cell line  (ATCC)


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    ATCC sf9 insect cell line
    Schematic representation of eIF3 complex expression and purification protocol. (a) Generation of baculovirus for eIF3 expression. <t>Sf9</t> cells are used to produce P0 and P1 viral progenies. Cell viability was assessed by trypan blue staining and bright field microscopy (top panels, dark cells correspond to death cells). Infected cells were identified based on YFP expression by fluorescence microscopy (red cells are infected cells). (b) Main protocol for purification of eIF3 complexes from over‐expressed in Hi5 insect cells. (c) Analysis of the integrity of purified eIF3 complex by analytical SEC. Chromatogram of SEC run using a Superose6 3.2/300 column of eIF3 <t>expressed</t> <t>in</t> <t>insect</t> cells and purified. Coomassie‐stained SDS polyacrylamide of the fractions indicate in the chromatogram.
    Sf9 Insect Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2365 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sf9 insect cell line/product/ATCC
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    sf9 insect cell line - by Bioz Stars, 2026-03
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    1) Product Images from "Purification and characterization of recombinant human translation initiation factor eIF3"

    Article Title: Purification and characterization of recombinant human translation initiation factor eIF3

    Journal: Protein Science : A Publication of the Protein Society

    doi: 10.1002/pro.70388

    Schematic representation of eIF3 complex expression and purification protocol. (a) Generation of baculovirus for eIF3 expression. Sf9 cells are used to produce P0 and P1 viral progenies. Cell viability was assessed by trypan blue staining and bright field microscopy (top panels, dark cells correspond to death cells). Infected cells were identified based on YFP expression by fluorescence microscopy (red cells are infected cells). (b) Main protocol for purification of eIF3 complexes from over‐expressed in Hi5 insect cells. (c) Analysis of the integrity of purified eIF3 complex by analytical SEC. Chromatogram of SEC run using a Superose6 3.2/300 column of eIF3 expressed in insect cells and purified. Coomassie‐stained SDS polyacrylamide of the fractions indicate in the chromatogram.
    Figure Legend Snippet: Schematic representation of eIF3 complex expression and purification protocol. (a) Generation of baculovirus for eIF3 expression. Sf9 cells are used to produce P0 and P1 viral progenies. Cell viability was assessed by trypan blue staining and bright field microscopy (top panels, dark cells correspond to death cells). Infected cells were identified based on YFP expression by fluorescence microscopy (red cells are infected cells). (b) Main protocol for purification of eIF3 complexes from over‐expressed in Hi5 insect cells. (c) Analysis of the integrity of purified eIF3 complex by analytical SEC. Chromatogram of SEC run using a Superose6 3.2/300 column of eIF3 expressed in insect cells and purified. Coomassie‐stained SDS polyacrylamide of the fractions indicate in the chromatogram.

    Techniques Used: Expressing, Purification, Staining, Microscopy, Infection, Fluorescence



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    Schematic representation of eIF3 complex expression and purification protocol. (a) Generation of baculovirus for eIF3 expression. <t>Sf9</t> cells are used to produce P0 and P1 viral progenies. Cell viability was assessed by trypan blue staining and bright field microscopy (top panels, dark cells correspond to death cells). Infected cells were identified based on YFP expression by fluorescence microscopy (red cells are infected cells). (b) Main protocol for purification of eIF3 complexes from over‐expressed in Hi5 insect cells. (c) Analysis of the integrity of purified eIF3 complex by analytical SEC. Chromatogram of SEC run using a Superose6 3.2/300 column of eIF3 <t>expressed</t> <t>in</t> <t>insect</t> cells and purified. Coomassie‐stained SDS polyacrylamide of the fractions indicate in the chromatogram.
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    Schematic representation of eIF3 complex expression and purification protocol. (a) Generation of baculovirus for eIF3 expression. <t>Sf9</t> cells are used to produce P0 and P1 viral progenies. Cell viability was assessed by trypan blue staining and bright field microscopy (top panels, dark cells correspond to death cells). Infected cells were identified based on YFP expression by fluorescence microscopy (red cells are infected cells). (b) Main protocol for purification of eIF3 complexes from over‐expressed in Hi5 insect cells. (c) Analysis of the integrity of purified eIF3 complex by analytical SEC. Chromatogram of SEC run using a Superose6 3.2/300 column of eIF3 <t>expressed</t> <t>in</t> <t>insect</t> cells and purified. Coomassie‐stained SDS polyacrylamide of the fractions indicate in the chromatogram.
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    Schematic representation of eIF3 complex expression and purification protocol. (a) Generation of baculovirus for eIF3 expression. Sf9 cells are used to produce P0 and P1 viral progenies. Cell viability was assessed by trypan blue staining and bright field microscopy (top panels, dark cells correspond to death cells). Infected cells were identified based on YFP expression by fluorescence microscopy (red cells are infected cells). (b) Main protocol for purification of eIF3 complexes from over‐expressed in Hi5 insect cells. (c) Analysis of the integrity of purified eIF3 complex by analytical SEC. Chromatogram of SEC run using a Superose6 3.2/300 column of eIF3 expressed in insect cells and purified. Coomassie‐stained SDS polyacrylamide of the fractions indicate in the chromatogram.

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Purification and characterization of recombinant human translation initiation factor eIF3

    doi: 10.1002/pro.70388

    Figure Lengend Snippet: Schematic representation of eIF3 complex expression and purification protocol. (a) Generation of baculovirus for eIF3 expression. Sf9 cells are used to produce P0 and P1 viral progenies. Cell viability was assessed by trypan blue staining and bright field microscopy (top panels, dark cells correspond to death cells). Infected cells were identified based on YFP expression by fluorescence microscopy (red cells are infected cells). (b) Main protocol for purification of eIF3 complexes from over‐expressed in Hi5 insect cells. (c) Analysis of the integrity of purified eIF3 complex by analytical SEC. Chromatogram of SEC run using a Superose6 3.2/300 column of eIF3 expressed in insect cells and purified. Coomassie‐stained SDS polyacrylamide of the fractions indicate in the chromatogram.

    Article Snippet: Sf9 insect cell line (ATCC, CRL‐1711) was used to generate the baculoviruses.

    Techniques: Expressing, Purification, Staining, Microscopy, Infection, Fluorescence